Rubber Seed Kernel as Potent Solid Substrate for the Production of Lipase by Pseudomonas aeruginosa Strain BUP2

Unni, K. N. and Faisal, Panichikkal Abdul and Priji, Prakasan and Sajith, Sreedharan and Sreedevi, Sreedharan and Hareesh, E. S. and Roy, Trikariyoor Asokan Nidheesh and Benjamin, Sailas (2015) Rubber Seed Kernel as Potent Solid Substrate for the Production of Lipase by Pseudomonas aeruginosa Strain BUP2. Advances in Enzyme Research, 03 (02). pp. 31-38. ISSN 2328-4846

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Abstract

This study explored the utility of flours of rubber seed, coconut and groundnut kernels, and de-oiled cakes of coconut and groundnut as solid substrate for the production of lipase by Pseudomonas aeruginosa strain BUP2 (MTCC No. 5924), a novel bacterium reported from the rumen of Malabari goat. Various proportions (10%, 20%, 30%, 40% or 50%) of flours or cakes were prepared (w/v) with BUP medium (pH 4, 5, 6, 7 or 8), and incubated at different temperature (25°C, 28°C, 30°C or 32°C) for 24 to 96 h. The samples were assayed for lipase activity at 24 h intervals. The rubber seed flour (20%)-BUP medium supported the maximum lipase production (871 U/gds) at 48h incubation (pH 6, 28°C), followed by ground nut flour (398 U/gds), while ground nut cake supported the least lipase production (244 U/gds). From this, it is evident that the cheaply available rubber seed is an efficient substrate for the production of lipase, irrespective of its known demerit that it contains the limarin, a toxin; in fact, we could not detect limarin in the fermented matter. Thus, the utility of rubber seed for the production of a costly enzyme is reported from a novel rumen bacterium, which would be advantageous for rubber farmers.

Item Type: Article
Subjects: European Repository > Chemical Science
Depositing User: Managing Editor
Date Deposited: 30 Jan 2023 04:38
Last Modified: 26 Feb 2024 03:56
URI: http://go7publish.com/id/eprint/1245

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