Study on Cloning, Sequencing and Phylogenetic Analysis of Granulocyte Macrophage Colony Stimulating Factor of Indian Cattle (Bos indicus)

Reverse transcriptase polymerase chain reaction (RT-PCR) was used to amplify the cDNA coding for Granulocyte macrophage colony stimulating factor (GM-CSF) from LPS activated macrophages, which was then cloned into the pCNDA vector and sequenced. Without the secretory signal peptide sequence, the amplified bovine GM-CSF cDNA was 755 bp long. The 755-bp open reading frame (ORF) coded for 143 amino acids with a molecular weight of 16200 Da, and it was 99 and 96 percent identical to Bos taurus and Bubalus bubalis, respectively. The phylogenetic analysis revealed swine and equine GM-CSF sequences are most different from buffalo, sheep, goat, and deer.