Experiments on Micellar Liquid Chromatographic Method for Simultaneous Determination of Atenolol and Aspirin in Bulk and Pharmaceutical Dosage Form

Micellar liquid chromatographic method has been developed for the simultaneous determination of
Atenolol and Aspirin in a bulk drug and pharmaceutical formulation. Chromatographic separation
achieved isocratically on ODS hypersil C18 column (5 μm, 250 mm × 4.6 mm) and micellar mobile
phase of 0.07M sodium dodecyl sulfate (SDS) pH 3 adjusted with phosphate buffer and 15% (v/v) 1-
propanol as organic modifier in the ratio 70: 30 v/v and ultraviolet detection at 225 nm are used for the
determination. Under these conditions, the studied Aspirin and Atenolol elute between 4.033 ± 0.02
and 6.042 ± 0.031 min at a 1 mL/min flow rate. Parameters such as linearity, precision, accuracy,
specificity and robustness are studied as reported in the ICH guidelines. Linearity for Atenolol and
Aspirin was in the range of 2-12 μg/mL and 4-24 μg/mL respectively. The mean recoveries obtained
for Atenolol and Aspirin were 99.55 and 99.61% respectively. Developed method was found to be
accurate, precise, selective and rapid for simultaneous estimation of Aspirin and Atenolol.