Enhanced Antioxidant and Anticancer Properties of Pulsed Electric Field-treated Black Grape Extract

Grape, one of the world’s largest fruit crops and most commonly consumed fruits in the world is rich
with antioxidant phytochemicals, such as polyphenols, which have both antioxidant and anticancer
properties. Grape extract comprises several bio-active compounds, mostly represented by
polyphenols and phenolic acids. It could be an excellent alternative to toxic chemo drugs, with least
side effects and with excellent cost/benefit ratio. Grape extract exerts high antioxidant and anticancer
activities against human cancer cell lines. It could be used to treat a number of cancers, including
lung, colon, cervical, bladder and prostate cancers. In this research, the antioxidant and anticancer
properties of pulsed electric field (PEF) treated grape extract is investigated. HeLa cell line (cervical
cancer) was used for this purpose. The results indicate more antioxidant activity and more cell death
due to PEF-treated extract compared to untreated one, indicating the enhanced potency of PEFtreated
extract. This shows the potential of using PEF-treated grape extract as a possible anti-cancer
drug.