CORRELATION OF CAPN10 POLYMORPHISM (rs3792267) WITH HYPERTENSION IN DIABETIC PATIENTS FROM IRAQ

Background: Calpain-10 is calcium-activated cysteine intracellular proteases, encoded by CAPN10. Recently, the CAPN10 Polymorphism (rs3792267) have been shown to be associated with type 2 diabetes mellitus (T2DM). The purpose of our study was to examine the association of this polymorphism (rs3792267) with coexisting hypertension in Iraqi individuals, affected by T2DM.

Methods: A case-control study was conducted, examining the association of the SNP, rs3792267 with T2DM, with and without hypertension. Of the total two hundred cases, 100 T2DM subjects with hypertension, and 100 T2DM individuals without hypertension, used as the patients and controls, respectively. They were selected randomly based on the WHO criteria. DNA was extracted from the blood, and genotyped by PCR-RFLP, using the restriction enzyme NdeI. Then, the PCR product was resolved on agarose gel electrophoresis.

Results: Analysis of the genotype and allele frequencies of CAPN10 polymorphism (rs3792267) revealed that the heterozygous genotype (AG) was significantly different (p=0.0001), while homozygous genotype (AA) shows no significant difference between the patient and control groups. The frequency of the A allele of the rs3792267 polymorphism was significantly different between the two groups (p=0.0001), 36.5% without hypertension and 15.5% with hypertension, (OR=0.1662, CI% 0.0901-0.3064), indicating that the A allele is associated with T2DM. On the other hand, no significant statistical difference was observed in the mean values of cholesterol and triglycerides of T2DM, with and without hypertension.

Conclusion: Although the polymorphism (rs3792267) in the CAPN10 appears to have a hereditary relationship with the pathogenesis of type 2 diabetic patients, but it was not linked with the increased risk of hypertension in T2DM.