In Vitro Flowering of Vitex negundo L. A Medicinal Plant

In vitro plant regeneration and flowering were achieved from two explants of Vitex negundo L on MS medium containing different concentrations of auxins and cytokinins. The highest percentage (95.3%) of flowering was noticed from nodal explants cultured on MS medium supplemented with BAP 2.0 mg/l and NAA 0.1 mg/l combination. Of the two explants tested, maximum number of flower buds (9.10 � 1.50) was obtained from nodal explants than shoot tip explants (7.83 �1.52). The regenerated shoots were transferred onto MS medium fortified with BAP (0.1 mg/l) and NAA (0.5 mg/l) for flower maturation and root induction. The regenerated plantlets have been successfully established in vermiculite soil. Subsequently, they were shifted to the field condition.