Effect of different Potting Mixtures on Acclimatization of In vitro Developed Plants on Survival Percentage under Glass House Condition

The experiment was conducted during the years 2020-22, all of the experiments for the current research were carried out in the Plant Tissue Culture Laboratory, Department of Plant Molecular Biology & Biotechnology, College of Agriculture, Gwalior, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior (M. P.). Pomegranate cv. Bhagwa plantlets were placed in polythene bags containing various potting mixtures, such as soil, FYM, and Vermicompost, alone and in various combinations. The hardened plantlets were then exposed to direct sunshine to help them acclimate to their new surroundings. The percentage of tissue culture plantlets that survived was calculated. Experiments were conducted in a Completely Randomized Design (CRD) with four replications of each treatment and each replication contain three explants. Data were analyzed using Duncan’s multiple range test whereas the survival percentages were analysed by arc-sine transformation. On the basis of present study it is concluded that cocopeat soil combination had the highest survival rate (95.00%), followed by cocopeat (85%). In the case of pomegranate, sand, perlite, vermiculite, vermiculite soil and perlite sand alone were not shown to be effective hardening materials because all of the plants withered within a week. After two months of hardening under polyhouse circumstances, cocopeat soil showed the greatest growth with a notable increase in plant height (37.23 cm), followed by cocopeat (33.03 cm). Therefore, we may conclude that cocopeat is the ideal material for pomegranate plants that have been produced in vitro.