Oluwajide, O. O. and Olowe, R. A. and Adefioye, O. J. and Adekunle, O. C. and Adeyemi, F. M. and Akinde, S. B. and Aluko, E.F and Olowe, O. A. (2024) Phenotypic and Genotypic Detection of UV-induced Bacteriocin Production in Enteric Gram-Negative Rods and their Activity against Escherichia coli O157: H7. International Journal of TROPICAL DISEASE & Health, 45 (6). pp. 21-31. ISSN 2278-1005
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Abstract
Background: Virulence and antibiotic resistance of bacterial pathogens are one of the most universal threats to public health care.
Materials: In this study, a total of two hundred and fifty-seven (257) enteric bacteria isolated from human rectal swabs and cattle dung were screened for production of low-toxic, high target-specific bactericidal macromolecule peptides (Bacteriocin) against strains of E. coli 0157: H7 using agar well diffusion method. The producer isolates were exposed to ultra-violet (UV) rays (UV 302nm and 254nm) for induction.
Results: Out of the 257 isolates, 21 (8.1%) were active against E. coli 0157: H7 due to their constitutive bacteriocin, however, on the induction by UV ray; only 7 (2.1%) retained their potency for bacteriocin production. Three (42.9%) of seven isolates were randomly selected for genotypic screening (Col 28b (L) and Col K genes) because of their increased zone of inhibition (bioactive ability) after exposure to UV ray; the isolates also showed resistance to ≤ 2 classes of antibiotics and exhibited Gamma/alpha haemolysis.
Conclusion: This study revealed Serratia marcescens in this work to possess an antimicrobial molecule of bacteriocinogenic type with possession of Col 28b gene.
Item Type: | Article |
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Subjects: | European Repository > Medical Science |
Depositing User: | Managing Editor |
Date Deposited: | 09 Apr 2024 05:37 |
Last Modified: | 09 Apr 2024 05:37 |
URI: | http://go7publish.com/id/eprint/4286 |